Electrical coupling between ventricular myocytes and myofibroblasts in the infarcted mouse heart.

ABSTRACT:

Aims

Recent studies have demonstrated electrotonic coupling between scar tissue and the surrounding myocardium in cryoinjured hearts. However, the electrical dynamics occurring at the myocyte-nonmyocyte interface in the fibrotic heart remain undefined. Here, we sought to develop an assay to interrogate the nonmyocyte cell type contributing to heterocellular coupling and to characterize, on a cellular scale, its voltage response in the infarct border zone of living hearts.

Methods and results

We used two-photon laser scanning microscopy in conjunction with a voltage-sensitive dye to record transmembrane voltage changes simultaneously from cardiomyocytes and adjoined nonmyocytes in Langendorff-perfused mouse hearts with healing myocardial infarction. Transgenic mice with cardi

SUBMITTER: Rubart M 

PROVIDER: S-EPMC6018934 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature