Genomewide phenotypic analysis of growth, cell morphogenesis, and cell cycle events in Escherichia coli.

ABSTRACT: Cell size, cell growth, and cell cycle events are necessarily intertwined to achieve robust bacterial replication. Yet, a comprehensive and integrated view of these fundamental processes is lacking. Here, we describe an image-based quantitative screen of the single-gene knockout collection of Escherichia coli and identify many new genes involved in cell morphogenesis, population growth, nucleoid (bulk chromosome) dynamics, and cell division. Functional analyses, together with high-dimensional classification, unveil new associations of morphological and cell cycle phenotypes with specific functions and pathways. Additionally, correlation analysis across ~4,000 genetic perturbations shows that growth rate is surprisingly not predictive of cell size. Growth rate was also uncorrelated w

SUBMITTER: Campos M 

PROVIDER: S-EPMC6018989 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature