Transgene-mediated post-transcriptional gene silencing is inhibited by 3' non-coding sequences in Paramecium.

ABSTRACT: Homology-dependent gene silencing is achieved in Paramecium by introduction of gene coding regions into the somatic nucleus at high copy number, resulting in reduced expression of all homologous genes. Although a powerful tool for functional analysis, the relationship of this phenomenon to gene silencing mechanisms in other organisms has remained obscure. We report here experiments using the T4a gene, a member of the trichocyst [corrected]matrix protein (TMP) multigene family encoding secretory proteins, and the ND7 gene, a single copy gene required for exocytotic membrane fusion. Silencing of either gene leads to an exocytosis-deficient phenotype easily scored on individual cells. For each gene we have tested the ability of different combinations of promoter, coding and 3' non-coding regi

SUBMITTER: Galvani A 

PROVIDER: S-EPMC60190 | biostudies-literature | 2001 Nov

REPOSITORIES: biostudies-literature