RNA sequencing confirms similarities between PPI-responsive oesophageal eosinophilia and eosinophilic oesophagitis.

ABSTRACT:

Background

Although current American guidelines distinguish proton pump inhibitor-responsive oesophageal eosinophilia (PPI-REE) from eosinophilic oesophagitis (EoE), these entities are broadly similar. While two microarray studies showed that they have similar transcriptomes, more extensive RNA sequencing studies have not been done previously.

Aim

To determine whether RNA sequencing identifies genetic markers distinguishing PPI-REE from EoE.

Methods

We retrospectively examined 13 PPI-REE and 14 EoE biopsies, matched for tissue eosinophil content, and 14 normal controls. Patients and controls were not PPI-treated at the time of biopsy. We did RNA sequencing on formalin-fixed, paraffin-embedded tissue, with differential expression confirmation by quantitative polymerase

SUBMITTER: Peterson KA 

PROVIDER: S-EPMC6019190 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature