Mapping phosphate modifications of substituted lipid A via a targeted MS³ CID/UVPD strategy.

ABSTRACT: Structural characterization of lipid A from Gram-negative bacteria remains a significant challenge, especially with respect to localizing modifications of the phosphate groups typically found on the reducing and non-reducing ends of the β-1',6-linked glucosamine disaccharide backbone of lipid A. As reported here, combining traditional collisional activated dissociation (CAD) and ultraviolet photodissociation (UVPD) in a hybrid MS3 approach facilitates identification and localization of substituents of the phosphate groups. The focus is on rapid identification and characterization of substituted lipid A species with specific emphasis on the modifications on the 1 and 4' phosphate moieties. Mapping these modifications, typically ones that modify the surface charges of lipopolysaccharides, is

SUBMITTER: Crittenden CM 

PROVIDER: S-EPMC6019210 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature