Mining Novel Constitutive Promoter Elements in Soil Metagenomic Libraries in Escherichia coli.

ABSTRACT: Although functional metagenomics has been widely employed for the discovery of genes relevant to biotechnology and biomedicine, its potential for assessing the diversity of transcriptional regulatory elements of microbial communities has remained poorly explored. Here, we experimentally mined novel constitutive promoter sequences in metagenomic libraries by combining a bi-directional reporter vector, high-throughput fluorescence assays and predictive computational methods. Through the expression profiling of fluorescent clones from two independent soil sample libraries, we have analyzed the regulatory dynamics of 260 clones with candidate promoters as a set of active metagenomic promoters in the host Escherichia coli. Through an in-depth analysis of selected clones, we were able to

SUBMITTER: Westmann CA 

PROVIDER: S-EPMC6019500 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature