Adult hepatocytes direct liver organogenesis through non-parenchymal cell recruitment in the kidney.

ABSTRACT:

Background & aims

Since the first account of the myth of Prometheus, the amazing regenerative capacity of the liver has fascinated researchers because of its enormous medical potential. Liver regeneration is promoted by multiple types of liver cells, including hepatocytes and liver non-parenchymal cells (NPCs), through complex intercellular signaling. However, the mechanism of liver organogenesis, especially the role of adult hepatocytes at ectopic sites, remains unknown. In this study, we demonstrate that hepatocytes alone spurred liver organogenesis to form an organ-sized complex 3D liver that exhibited native liver architecture and functions in the kidneys of mice.

Methods

Isolated hepatocytes were transplanted under the kidney capsule of monocrotaline (MCT) and partial h

SUBMITTER: Utoh R 

PROVIDER: S-EPMC6019609 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature