Computational identification and evolutionary analysis of toxins in Mosquitocidal Bacillus thuringiensis strain S2160-1.

ABSTRACT: Mosquitocidal Bacillus thuringiensis (Bt) strain S2160-1 was proposed to be an alternative to Bacillus thuringiensis subsp. israelensis (Bti). Discovering and validating a toxic gene by experimentation was a complex and time-consuming task, which can benefit from high-throughput sequencing analysis. In this research, we predicted and identified toxic proteins in the strain S2160-1 based on the draft whole genome sequence data. Through a local BLASP, 46 putative toxins were identified in S2160-1 genome, by searching against a customized B. thuringiensis toxin proteins database containing 653 protein or peptide sequences retrieved from public accessible resources and PCR/clone results in our laboratory (e value = 1e - 5). These putative toxins

SUBMITTER: Liu P 

PROVIDER: S-EPMC6019651 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature