Characterization of a 16SrII subgroup D phytoplasma strain associated with Calendula officinalis phyllody in Iran.

ABSTRACT: Calendula officinalis plants with phyllody symptoms (CaoP) were observed in Yazd and Ashkezar (Yazd province, Iran) during 2013-2016. Twenty-one symptomatic and four asymptomatic plants were transferred individually to the greenhouse and potted for the biological and molecular characterization of associated phytoplasma. The dodder transmission from symptomatic potted marigold plants, induced virescence, phyllody and witches' broom symptoms in periwinkle. Total DNAs extracted from symptomatic and symptomless plants and dodder-inoculated periwinkles were subjected to nested PCR assay using primer pairs amplifying phytoplasma ribosomal DNA. Expected PCR amplification was detected in all CaoP plant and dodder-inoculated periwinkle samples. RFLP analysis of the amplicons obtained in dire

SUBMITTER: Esmailzadeh Hosseini SA 

PROVIDER: S-EPMC6019654 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature