Development and validation of an integrated DNA walking strategy to detect GMO expressing cry genes.

ABSTRACT:

Background

Recently, an integrated DNA walking strategy has been proposed to prove the presence of GMO via the characterisation of sequences of interest, including their transgene flanking regions and the unnatural associations of elements in their transgenic cassettes. To this end, the p35S, tNOS and t35S pCAMBIA elements have been selected as key targets, allowing the coverage of most of GMO, EU authorized or not. In the present study, a bidirectional DNA walking method anchored on the CryAb/c genes is proposed with the aim to cover additional GMO and additional sequences of interest.

Results

The performance of the proposed bidirectional DNA walking method anchored on the CryAb/c genes has been evaluated in a first time for its feasibility using several GM events possessin

SUBMITTER: Fraiture MA 

PROVIDER: S-EPMC6020286 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature