Efficient heterologous expression of an alkaline lipase and its application in hydrolytic production of free astaxanthin.

ABSTRACT:

Background

Astaxanthin, a naturally occurring carotenoid pigment molecule, displays strong antioxidant, anti-cancer, and immunity-enhancing properties, and is often utilized in food, biomedical, cosmetic, and other industries. Free astaxanthin has better solubility than astaxanthin esters (Ast-E), and is a useful auxiliary ingredient in health foods and medicines. Our goal was to establish an improved enzymatic method for preparation of free astaxanthin from natural sources (e.g., the microalga Haematococcus pluvialis), to expand the potential applications of free astaxanthin.

Results

The alkaline lipase gene proalip and its propeptide were cloned and successfully fusion-expressed in Pichia pastoris X-33. The recombinant lipase was termed Lipase-YH. Thro

SUBMITTER: Huang J 

PROVIDER: S-EPMC6020301 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature