Dataset Information

Post-translational buffering leads to convergent protein expression levels between primates.

ABSTRACT:

Background

Differences in gene regulation between human and closely related species influence phenotypes that are distinctly human. While gene regulation is a multi-step process, the majority of research concerning divergence in gene regulation among primates has focused on transcription.

Results

To gain a comprehensive view of gene regulation, we surveyed genome-wide ribosome occupancy, which reflects levels of protein translation, in lymphoblastoid cell lines derived from human, chimpanzee, and rhesus macaque. We further integrated messenger RNA and protein level measurements collected from matching cell lines. We find that, in addition to transcriptional regulation, the major factor determining protein level divergence between human and closely related species is post-translational buffering. Inter-species divergence in transcription is generally propagated to the level of protein translation. In contrast, gene expression divergence is often attenuated post-translationally, potentially mediated through post-translational modifications.

Conclusions

Results from our analysis indicate that post-translational buffering is a conserved mechanism that led to relaxation of selective constraint on transcript levels in humans.

SUBMITTER: Wang SH

PROVIDER: S-EPMC6020354 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature

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Publications

Post-translational buffering leads to convergent protein expression levels between primates.

Wang Sidney H SH Hsiao Chiaowen Joyce CJ Khan Zia Z Pritchard Jonathan K JK

Genome biology 20180627 1

<h4>Background</h4>Differences in gene regulation between human and closely related species influence phenotypes that are distinctly human. While gene regulation is a multi-step process, the majority of research concerning divergence in gene regulation among primates has focused on transcription.<h4>Results</h4>To gain a comprehensive view of gene regulation, we surveyed genome-wide ribosome occupancy, which reflects levels of protein translation, in lymphoblastoid cell lines derived from human, ...[more]

PMID: 29950183

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Dataset Information

Post-translational buffering leads to convergent protein expression levels between primates.

Background

Results

Conclusions

Publications

Post-translational buffering leads to convergent protein expression levels between primates.

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