Therapy-induced stress response is associated with downregulation of pre-mRNA splicing in cancer cells.

ABSTRACT:

Background

Abnormal pre-mRNA splicing regulation is common in cancer, but the effects of chemotherapy on this process remain unclear.

Methods

To evaluate the effect of chemotherapy on slicing regulation, we performed meta-analyses of previously published transcriptomic, proteomic, phosphoproteomic, and secretome datasets. Our findings were verified by LC-MS/MS, western blotting, immunofluorescence, and FACS analyses of multiple cancer cell lines treated with cisplatin and pladienolide B.

Results

Our results revealed that different types of chemotherapy lead to similar changes in alternative splicing by inducing intron retention in multiple genes. To determine the mechanism underlying this effect, we analyzed gene expression in 101 cell lines affected by ɣ-irradiation,

SUBMITTER: Anufrieva KS 

PROVIDER: S-EPMC6020472 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature