NIR in, far-red out: developing a two-photon fluorescent probe for tracking nitric oxide in deep tissue.

ABSTRACT: As a pivotal signalling molecule involved in various physiological and pathological processes, nitric oxide (NO) has motivated increasing interest in the last few decades. Although a considerable number of fluorescent probes have been developed for NO imaging, the in situ tracking of this gas molecule in biological events remains a big challenge, mainly because of the relatively short excitation and/or emission wavelengths, which are subject to background interference and lowered collection efficiency in deep-tissue imaging. Herein, we report a far-red emissive (650 nm) two-photon (TP) excitable NRNO probe, using Nile Red as the TP fluorophore, for NO detection and imaging both in vitro and in vivo. The NRNO probe shows a fast (within 180 s) and specific

SUBMITTER: Mao Z 

PROVIDER: S-EPMC6020528 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature