Optimized retroviral transduction of mouse T cells for in vivo assessment of gene function.

ABSTRACT: Retroviral (RV) expression of genes of interest (GOIs) is an invaluable tool and has formed the foundation of cellular engineering for adoptive cell therapy in cancer and other diseases. However, monitoring of transduced T cells long term (weeks to months) in vivo remains challenging because of the low frequency and often poor durability of transduced T cells over time when transferred without enrichment. Traditional methods often require additional overnight in vitro culture after transduction. Moreover, in vitro-generated effector CD8⁺ T cells enriched by sorting often have reduced viability, making it difficult to monitor the fate of transferred cells in vivo. Here, we describe an optimized mouse CD8⁺ T-cell RV transduction protocol that uses simple and rapid Perco

SUBMITTER: Kurachi M 

PROVIDER: S-EPMC6020692 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature