Optimizing Crystal Size of Photosystem II by Macroseeding: Toward Neutron Protein Crystallography.

ABSTRACT: Photosystem II (PSII) catalyzes the photo-oxidation of water to molecular oxygen and protons. The water splitting reaction occurs inside the oxygen-evolving complex (OEC) via a Mn₄CaO₅ cluster. To elucidate the reaction mechanism, detailed structural information for each intermediate state of the OEC is required. Despite the current high-resolution crystal structure of PSII at 1.85 Å and other efforts to follow the structural changes of the Mn₄CaO₅ cluster using X-ray free electron laser (XFEL) crystallography in addition to spectroscopic methods, many details about the reaction mechanism and conformational changes in the catalytic site during water oxidation still remain elusive. In this study, we present a rarely found successful application of

SUBMITTER: Hussein R 

PROVIDER: S-EPMC6020701 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature