A Predictive 7-Gene Assay and Prognostic Protein Biomarkers for Non-small Cell Lung Cancer.

ABSTRACT:

Purpose

This study aims to develop a multi-gene assay predictive of the clinical benefits of chemotherapy in non-small cell lung cancer (NSCLC) patients, and substantiate their protein expression as potential therapeutic targets.

Patients and methods

The mRNA expression of 160 genes identified from microarray was analyzed in qRT-PCR assays of independent 337 snap-frozen NSCLC tumors to develop a predictive signature. A clinical trial JBR.10 was included in the validation. Hazard ratio was used to select genes, and decision-trees were used to construct the predictive model. Protein expression was quantified with AQUA in 500 FFPE NSCLC samples.

Results

A 7-gene signature was identified from training cohort (n = 83) with accurate patient stratification (P = 0.0043) and w

SUBMITTER: Guo NL 

PROVIDER: S-EPMC6020749 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature