Quantitative analysis of focal adhesion dynamics using photonic resonator outcoupler microscopy (PROM).

ABSTRACT: Focal adhesions are critical cell membrane components that regulate adhesion and migration and have cluster dimensions that correlate closely with adhesion engagement and migration speed. We utilized a label-free approach for dynamic, long-term, quantitative imaging of cell-surface interactions called photonic resonator outcoupler microscopy (PROM) in which membrane-associated protein aggregates outcoupled photons from the resonant evanescent field of a photonic crystal biosensor, resulting in a highly localized reduction of the reflected light intensity. By mapping the changes in the resonant reflected peak intensity from the biosensor surface, we demonstrate the ability of PROM to detect focal adhesion dimensions. Similar spatial distributions can be observed between PROM images and fluo

SUBMITTER: Zhuo Y 

PROVIDER: S-EPMC6020849 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature