A Cdk9-PP1 switch regulates the elongation-termination transition of RNA polymerase II.

ABSTRACT: The end of the RNA polymerase II (Pol II) transcription cycle is strictly regulated to prevent interference between neighbouring genes and to safeguard transcriptome integrity ¹ . The accumulation of Pol II downstream of the cleavage and polyadenylation signal can facilitate the recruitment of factors involved in mRNA 3'-end formation and termination ² , but how this sequence is initiated remains unclear. In a chemical-genetic screen, human protein phosphatase 1 (PP1) isoforms were identified as substrates of positive transcription elongation factor b (P-TEFb), also known as the cyclin-dependent kinase 9 (Cdk9)-cyclin T1 (CycT1) complex ³ . Here we show that Cdk9 and PP1 govern phosphorylation of the conserved elongation factor Spt5 in the fission yeast Sch

SUBMITTER: Parua PK 

PROVIDER: S-EPMC6021199 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature