Extracellular vesicles generated by placental tissues ex vivo: A transport system for immune mediators and growth factors.

ABSTRACT:

Problem

To study the mechanisms of placenta function and the role of extracellular vesicles (EVs) in pregnancy, it is necessary to develop an ex vivo system that retains placental cytoarchitecture and the primary metabolic aspects, in particular the release of EVs and soluble factors. Here, we developed such a system and investigated the pattern of secretion of cytokines, growth factors, and extracellular vesicles by placental villous and amnion tissues ex vivo.

Methods of study

Placental villous and amnion explants were cultured for 2 weeks at the air/liquid interface and their morphology and the released cytokines and EVs were analyzed. Cytokines were analyzed with multiplexed bead assays, and individual EVs were analyzed with recently developed techniques that involved EV

SUBMITTER: Fitzgerald W 

PROVIDER: S-EPMC6021205 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature