Influence of Vitamin D Deficiency on Progression of Experimental Otitis Media in Rats.

ABSTRACT:

Background

Vitamin D plays an important role in the immune response against infection. The purpose of the present study was to investigate the influence of vitamin D deficiency on the progression of otitis media (OM) using an experimental rat model.

Methods

Four-week-old male Sprague-Dawley rats (n=72) were divided into two groups based on their diet: a control diet group (n=36) and a vitamin D-deficient diet group (n=36). After 8 weeks of diet, experimental OM was induced by inoculation of non-typeable Haemophilus influenzae in the middle ear cavity. The rats were evaluated with otomicroscopy to determine the inflammation in the middle ear mucosa on days 1, 2, 4, 7, and 14 post-inoculation. Bullae from sacrificed rats were collected and analyzed histologically.

Results</

SUBMITTER: Kim HB 

PROVIDER: S-EPMC6021308 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature