A novel sample preparation strategy for shotgun lipidomics of phospholipids employing multilamellar vesicles.

ABSTRACT: The identification of lipids in biological samples is gaining importance. The advent of mass spectrometry-based lipidomics accelerated the field allowing nowadays for identification and quantification of complete lipidomes. However, due to solubility difficulties and varying properties of different lipid classes, sample preparation for lipidomics is still an issue. Of the many lipid classes, phospholipids are the major components of biological membranes. In solution, they spontaneously form lipid vesicles of various structures such as liposomes. They are therefore often used as membrane mimics when studying biological membranes and membrane proteins. Here, we present a novel sample preparation strategy for shotgun lipidomics employing liposomes prepared from lipid standards or lipid mixtur

SUBMITTER: Frick M 

PROVIDER: S-EPMC6021460 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature