The contribution of lot-to-lot variation to the measurement uncertainty of an LC-MS-based multi-mycotoxin assay.

ABSTRACT: Multi-mycotoxin determination by LC-MS is commonly based on external solvent-based or matrix-matched calibration and, if necessary, the correction for the method bias. In everyday practice, the method bias (expressed as apparent recovery RA), which may be caused by losses during the recovery process and/or signal/suppression enhancement, is evaluated by replicate analysis of a single spiked lot of a matrix. However, RA may vary for different lots of the same matrix, i.e., lot-to-lot variation, which can result in a higher relative expanded measurement uncertainty (U _r ). We applied a straightforward procedure for the calculation of U _r from the within-laboratory reproducibility, which is also called intermediate precision, and the uncertainty of RA (u_r,RA).

SUBMITTER: Stadler D 

PROVIDER: S-EPMC6021480 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature