Solvatochromic fluorene-linked nucleoside and DNA as color-changing fluorescent probes for sensing interactions.

ABSTRACT: A nucleoside bearing a solvatochromic push-pull fluorene fluorophore (dC^FL ) was designed and synthesized by the Sonogashira coupling of alkyne-linked fluorene 8 with 5-iodo-2'-deoxycytidine. The fluorene building block 8 and labeled nucleoside dC^FL exerted bright fluorescence with significant solvatochromic effect providing emission maxima ranging from 421 to 544 nm and high quantum yields even in highly polar solvents, including water. The solvatochromism of 8 was studied by DFT and ADC(2) calculations to show that, depending on the polarity of the solvent, emission either from the planar or the twisted conformation of the excited state can occur. The nucleoside was converted to its triphosphate variant dC^FLTP whi

SUBMITTER: Dziuba D 

PROVIDER: S-EPMC6021979 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature