Paternal sepsis induces alterations of the sperm methylome and dampens offspring immune responses-an animal study.

ABSTRACT:

Background

Sepsis represents the utmost severe consequence of infection, involving a dysregulated and self-damaging immune response of the host. While different environmental exposures like chronic stress or malnutrition have been well described to reprogram the germline and subsequently offspring attributes, the intergenerational impact of sepsis as a tremendous immunological stressor has not been examined yet.

Methods

Polymicrobial sepsis in 12-week-old male C57BL/6 mice was induced by cecal ligation and puncture (CLP), followed by a mating of the male survivors (or appropriate sham control animals) 6 weeks later with healthy females. Alveolar macrophages of offspring animals were isolated and stimulated with either LPS or Zymosan, and supernatant levels of TNF-α were quan

SUBMITTER: Bomans K 

PROVIDER: S-EPMC6022485 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature