Molecular cloning and characterization of the endothelin 3 gene in black bone sheep.

ABSTRACT:

Background

Black bone sheep was first discovered in Yunnan province of China in 1970, with unique black pigmentation on the body and internal organs. Endothelin 3 (EDN3) has been known as a key gene causing hyperpigmentation in black bone chicken, the Silky fowl.

Methods

In this study, EDN3 was employed as a candidate gene for regulating black color pigmentation. First, EDN3 was cloned from sheep to obtain the full-length cDNA by using the rapid amplification of cDNA ends (RACE). Genomic EDN3 was screened and a total of thirty predicted single nucleotide polymorphisms (SNPs) were genotyped for allele and genotype frequency analysis in a case-control study involving two black bone sheep populations. Genomic copy number analysis of EDN3 in sh

SUBMITTER: Darwish HYA 

PROVIDER: S-EPMC6022492 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature