A spectral clustering-based method for identifying clones from high-throughput B cell repertoire sequencing data.

ABSTRACT:

Motivation

B cells derive their antigen-specificity through the expression of Immunoglobulin (Ig) receptors on their surface. These receptors are initially generated stochastically by somatic re-arrangement of the DNA and further diversified following antigen-activation by a process of somatic hypermutation, which introduces mainly point substitutions into the receptor DNA at a high rate. Recent advances in next-generation sequencing have enabled large-scale profiling of the B cell Ig repertoire from blood and tissue samples. A key computational challenge in the analysis of these data is partitioning the sequences to identify descendants of a common B cell (i.e. a clone). Current methods group sequences using a fixed distance threshold, or a likelihood calculation that is computati

SUBMITTER: Nouri N 

PROVIDER: S-EPMC6022594 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature