IDP-denovo: de novo transcriptome assembly and isoform annotation by hybrid sequencing.

ABSTRACT:

Motivation

In the past years, the long read (LR) sequencing technologies, such as Pacific Biosciences and Oxford Nanopore Technologies, have been demonstrated to substantially improve the quality of genome assembly and transcriptome characterization. Compared to the high cost of genome assembly by LR sequencing, it is more affordable to generate LRs for transcriptome characterization. That is, when informative transcriptome LR data are available without a high-quality genome, a method for de novo transcriptome assembly and annotation is of high demand.

Results

Without a reference genome, IDP-denovo performs de novo transcriptome assembly, isoform annotation and quantification by integrating the strengths of LRs and short reads. Using the GM12878 human data as a gold standard

SUBMITTER: Fu S 

PROVIDER: S-EPMC6022631 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature