Viral quasispecies reconstruction via tensor factorization with successive read removal.

ABSTRACT:

Motivation

As RNA viruses mutate and adapt to environmental changes, often developing resistance to anti-viral vaccines and drugs, they form an ensemble of viral strains--a viral quasispecies. While high-throughput sequencing (HTS) has enabled in-depth studies of viral quasispecies, sequencing errors and limited read lengths render the problem of reconstructing the strains and estimating their spectrum challenging. Inference of viral quasispecies is difficult due to generally non-uniform frequencies of the strains, and is further exacerbated when the genetic distances between the strains are small.

Results

This paper presents TenSQR, an algorithm that utilizes tensor factorization framework to analyze HTS data and reconstruct viral quasispecies characterized by highly uneven

SUBMITTER: Ahn S 

PROVIDER: S-EPMC6022648 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature