Versatile genome assembly evaluation with QUAST-LG.

ABSTRACT:

Motivation

The emergence of high-throughput sequencing technologies revolutionized genomics in early 2000s. The next revolution came with the era of long-read sequencing. These technological advances along with novel computational approaches became the next step towards the automatic pipelines capable to assemble nearly complete mammalian-size genomes.

Results

In this manuscript, we demonstrate performance of the state-of-the-art genome assembly software on six eukaryotic datasets sequenced using different technologies. To evaluate the results, we developed QUAST-LG-a tool that compares large genomic de novo assemblies against reference sequences and computes relevant quality metrics. Since genomes generally cannot be reconstructed completely due to complex repeat patterns a

SUBMITTER: Mikheenko A 

PROVIDER: S-EPMC6022658 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature