Physiological performance of transplastomic tobacco plants overexpressing aquaporin AQP1 in chloroplast membranes.

ABSTRACT: The leaf mesophyll CO2 conductance and the concentration of CO2 within the chloroplast are major factors affecting photosynthetic performance. Previous studies have shown that the aquaporin NtAQP1 (which localizes to the plasma membrane and chloroplast inner envelope membrane) is involved in CO2 permeability in the chloroplast. Levels of NtAQP1 in plants genetically engineered to overexpress the protein correlated positively with leaf mesophyll CO2 conductance and photosynthetic rate. In these studies, the nuclear transformation method used led to changes in NtAQP1 levels in the plasma membrane and the chloroplast inner envelope membrane. In the present work, NtAQP1 levels were increased up to 16-fold in the chloroplast membranes alone by the overexpression of NtAQP1 from the plastid genom

SUBMITTER: Fernandez-San Millan A 

PROVIDER: S-EPMC6022695 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature