Complex characterization of oat (Avena sativa L.) lines obtained by wide crossing with maize (Zea mays L.).

ABSTRACT:

Background

The oat × maize addition (OMA) lines are used for mapping of the maize genome, the studies of centromere-specific histone (CENH3), gene expression, meiotic chromosome behavior and also for introducing maize C4 photosynthetic system to oat. The aim of our study was the identification and molecular-cytogenetic characterization of oat × maize hybrids.

Methods

Oat DH lines and oat × maize hybrids were obtained using the wide crossing of Avena sativa L. with Zea mays L. The plants identified as having a Grande-1 retrotransposon fragment, which produced seeds, were used for genomic in situ hybridization (GISH).

Results

A total of 138 oat lines obtained by crossing of 2,314 oat plants from 80 genotypes with maize cv. Waza were tested fo

SUBMITTER: Skrzypek E 

PROVIDER: S-EPMC6022724 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature