Modeling Structure and Dynamics of Protein Complexes with SAXS Profiles.

ABSTRACT: Small-angle X-ray scattering (SAXS) is an increasingly common and useful technique for structural characterization of molecules in solution. A SAXS experiment determines the scattering intensity of a molecule as a function of spatial frequency, termed SAXS profile. SAXS profiles can be utilized in a variety of molecular modeling applications, such as comparing solution and crystal structures, structural characterization of flexible proteins, assembly of multi-protein complexes, and modeling of missing regions in the high-resolution structure. Here, we describe protocols for modeling atomic structures based on SAXS profiles. The first protocol is for comparing solution and crystal structures including modeling of missing regions and determination of the oligomeric state. The second protocol

SUBMITTER: Schneidman-Duhovny D 

PROVIDER: S-EPMC6022765 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature