Secretion, isotopic labeling and deglycosylation of N-acylethanolamine acid amidase for biophysical studies.

ABSTRACT: N-acylethanolamine acid amidase (NAAA) is an N-terminal nucleophile (Ntn) enzyme with a catalytic cysteine residue that has highest activity at acidic pH. The most prominent substrate hydrolyzed is palmitoylethanolamine (PEA), which regulates inflammation. Inhibitors of NAAA have been shown to increase endogenous levels of PEA, and are of interest as potential treatments for inflammatory disorders and other maladies. Currently, there are no X-ray or NMR structures of NAAA available to inform medicinal chemistry. Additionally, there are a limited number of enzyme structures available that are within the Ntn-hydrolase family, have a catalytic cysteine residue, and have a high sequence homology. For these reasons, we developed expression and purification methods for the production of enzyme s

SUBMITTER: Pavlopoulos S 

PROVIDER: S-EPMC6022831 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature