Ontology highlight
ABSTRACT:
SUBMITTER: Ma S
PROVIDER: S-EPMC6023403 | biostudies-literature | 2018 Mar
REPOSITORIES: biostudies-literature
Nature biomedical engineering 20180307 3
Methylomic analyses typically require substantial amounts of DNA, thus hindering studies involving scarce samples. Here, we show that microfluidic diffusion-based reduced representative bisulfite sequencing (MID-RRBS) permits high-quality methylomic profiling with nanogram-to-single-cell quantities of starting DNA. We used the microfluidic device, which allows for efficient bisulfite conversion with high DNA recovery, to analyse genome-wide DNA methylation in cell nuclei isolated from mouse brai ...[more]