Oxidative stress induces p38MAPK-dependent senescence in the feto-maternal interface cells.

ABSTRACT: OBJECTIVE:This study tested the mechanism of the oxidative stress (OS)-induced senescence pathway at the feto-maternal interface cells. METHODS:Primary amnion mesenchymal cells (AMCs), chorion and decidual cells isolated from the placental membranes of women at normal term (not in labor) were exposed to OS-inducing cigarette smoke extract (CSE) for 48?h. Reactive oxygen species (ROS) was measured using 2'7'-dichlorodihydrofluorescein. Western blot analysis determined phosphorylated (P) p38MAPK and p53 expression. Senescence-associated ?-Galactosidase (SA-?-Gal) and matrix metallopeptidase 9 (MMP9) histochemistry were used to measure senescence and inflammation respectively. Cotreatment of cells with the antioxidant, N-acetyl cysteine (NAC), or the p38MAPK inhibitor, SB203580 (SB), verified the activation specificity. RESULTS:CSE increased ROS production from AMCs, chorion cells, and decidual cells (P?

SUBMITTER: Jin J 

PROVIDER: S-EPMC6023622 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature