Salivary exRNA biomarkers to detect gingivitis and monitor disease regression.

ABSTRACT:

Aim

This study tests the hypothesis that salivary extracellular RNA (exRNA) biomarkers can be developed for gingivitis detection and monitoring disease regression.

Materials and methods

Salivary exRNA biomarker candidates were developed from a total of 100 gingivitis and non-gingivitis individuals using Affymetrix's expression microarrays. The top 10 differentially expressed exRNAs were tested in a clinical cohort to determine whether the discovered salivary exRNA markers for gingivitis were associated with clinical gingivitis and disease regression. For this purpose, unstimulated saliva was collected from 30 randomly selected gingivitis subjects, the gingival and plaque indexes scores were taken at baseline, 3 and 6 weeks and salivary exRNAs were assayed by means of reverse

SUBMITTER: Kaczor-Urbanowicz KE 

PROVIDER: S-EPMC6023773 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature