Self-Amplifying Replicon RNA Delivery to Dendritic Cells by Cationic Lipids.

ABSTRACT: Advances in RNA technology during the past two decades have led to the construction of replication-competent RNA, termed replicons, RepRNA, or self-amplifying mRNA, with high potential for vaccine applications. Cytosolic delivery is essential for their translation and self-replication, without infectious progeny generation, providing high levels of antigen expression for inducing humoral and cellular immunity. Synthetic nanoparticle-based delivery vehicles can both protect the RNA molecules and facilitate targeting of dendritic cells-critical for immune defense development. Several cationic lipids were assessed, with RepRNA generated from classical swine fever virus encoding nucleoprotein genes of influenza A virus. The non-cytopathogenic nature of the RNA allowed targeting to dendritic ce

SUBMITTER: Englezou PC 

PROVIDER: S-EPMC6023837 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature