Human CD8⁺CD28^- T Suppressor Cells Expanded by IL-15 In Vitro Suppress in an Allospecific and Programmed Cell Death Protein 1-Dependent Manner.

ABSTRACT: CD8⁺CD28^- T suppressor cells (Ts) have been recently documented to play an important role in alloimmunity. Therefore, understanding and optimizing the conditions under which these cells are generated and/or expanded would greatly facilitate further research and potential clinical use. In this study, we describe rapid expansion of human allospecific CD8⁺CD28^- Ts cells through coculture of CD8⁺ T cells with human leukocyte antigen-mismatched donor antigen-presenting cells plus IL-15 in a relative short period of time in vitro. Interestingly, IL-15 promotes the expansion of CD8⁺CD28^- Ts cells through several parallel mechanisms. The expanded CD8⁺CD28^- Ts cells upregulate expression of CD1

SUBMITTER: Feng F 

PROVIDER: S-EPMC6023977 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature