Applying DNA rolling circle amplification in fluorescence imaging of cell surface glycans labeled by a metabolic method.

ABSTRACT: Glycans on the cell surfaces are essential for cellular communication. Metabolically labeling glycans can introduce unnatural sugars into cellular glycans, which can facilitate further labeling. We report herein imaging cell surface glycosylation by using click chemistry and DNA rolling circle amplification (RCA) to improve detection sensitivity. Through the RCA amplification, the image resolution of a cell was significantly improved and much fewer unnatural sugars were used than required previously. The advantage of this method is that it avoids introducing too much unnatural sugar, which can interfere with normal, physiological cell function. Simultaneously, the enhanced fluorescence intensity conveniently facilitates the detection of cells' own biosynthetic glycans by simply using a mic

SUBMITTER: Zhang X 

PROVIDER: S-EPMC6024553 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature