Retinal Degeneration Triggers the Activation of YAP/TEAD in Reactive Muller Cells.

ABSTRACT:

Purpose

During retinal degeneration, Müller glia cells respond to photoreceptor loss by undergoing reactive gliosis, with both detrimental and beneficial effects. Increasing our knowledge of the complex molecular response of Müller cells to retinal degeneration is thus essential for the development of new therapeutic strategies. The purpose of this work was to identify new factors involved in Müller cell response to photoreceptor cell death.

Methods

Whole transcriptome sequencing was performed from wild-type and degenerating rd10 mouse retinas at P30. The changes in mRNA abundance for several differentially expressed genes were assessed by quantitative RT-PCR (RT-qPCR). Protein expression level and retinal cellular localization were determined by western blot and immunohisto

SUBMITTER: Hamon A 

PROVIDER: S-EPMC6024660 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature