SINHCAF/FAM60A and SIN3A specifically repress HIF-2? expression.

ABSTRACT: The SIN3A-HDAC (histone deacetylase) complex is a master transcriptional repressor, required for development but often deregulated in disease. Here, we report that the recently identified new component of this complex, SINHCAF (SIN3A and HDAC-associated factor)/FAM60A (family of homology 60A), links the SIN3A-HDAC co-repressor complex function to the hypoxia response. We show that SINHCAF specifically represses HIF-2? mRNA and protein expression, via its interaction with the transcription factor SP1 (specificity protein 1) and recruitment of HDAC1 to the HIF-2? promoter. SINHCAF control over HIF-2? results in functional cellular changes in in vitro angiogenesis and viability. Our analysis reveals an unexpected link between SINHCAF and the regulation of the hypoxia response.

SUBMITTER: Biddlestone J 

PROVIDER: S-EPMC6024822 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature