Evaluating the Performance of De Novo Assembly Methods for Venom-Gland Transcriptomics.

ABSTRACT: Venom-gland transcriptomics is a key tool in the study of the evolution, ecology, function, and pharmacology of animal venoms. In particular, gene-expression variation and coding sequences gained through transcriptomics provide key information for explaining functional venom variation over both ecological and evolutionary timescales. The accuracy and usefulness of inferences made through transcriptomics, however, is limited by the accuracy of the transcriptome assembly, which is a bioinformatic problem with several possible solutions. Several methods have been employed to assemble venom-gland transcriptomes, with the Trinity assembler being the most commonly applied among them. Although previous evidence of variation in performance among assembly software exists, particularly regarding rec

SUBMITTER: Holding ML 

PROVIDER: S-EPMC6024825 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature