Structural Characterisation of Predicted Helical Regions in the Chironex fleckeri CfTX-1 Toxin.

ABSTRACT: The Australian jellyfish Chironex fleckeri, belongs to a family of cubozoan jellyfish known for their potent venoms. CfTX-1 and -2 are two highly abundant toxins in the venom, but there is no structural data available for these proteins. Structural information on toxins is integral to the understanding of the mechanism of these toxins and the development of an effective treatment. Two regions of CfTX-1 have been predicted to have helical structures that are involved with the mechanism of action. Here we have synthesized peptides corresponding to these regions and analyzed their structures using NMR spectroscopy. The peptide corresponding to the predicted N-terminal amphiphilic helix appears unstructured in aqueous solution. This lack of structure concurs with structural disorder pre

SUBMITTER: Andreosso A 

PROVIDER: S-EPMC6024933 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature