A Marine Isolate of Bacillus pumilus Secretes a Pumilacidin Active against Staphylococcus aureus.

ABSTRACT: Producing antimicrobials is a common adaptive behavior shared by many microorganisms, including marine bacteria. We report that SF214, a marine-isolated strain of Bacillus pumilus, produces at least two different molecules with antibacterial activity: a molecule smaller than 3 kDa active against Staphylococcus aureus and a molecule larger than 10 kDa active against Listeria monocytogenes. We focused our attention on the anti-Staphylococcus molecule and found that it was active at a wide range of pH conditions and that its secretion was dependent on the growth phase, medium, and temperature. A mass spectrometry analysis of the size-fractionated supernatant of SF214 identified the small anti-Staphylococcus molecule as a pumilacidin, a nonribosomally synthesized biosurfactant composed of a mi

SUBMITTER: Saggese A 

PROVIDER: S-EPMC6025400 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature