Development of novel EST-SSR markers for Rhododendron longipedicellatum (Ericaceae) and cross-amplification in two congeners.

ABSTRACT:

Premise of the study

To investigate the genetic background and population characteristics of Rhododendron longipedicellatum (Ericaceae), a newly discovered and critically endangered species, expressed sequence tag-simple sequence repeat markers were developed, and transferability was tested in two congeners, R. molle and R. simsii.

Methods and results

Based on the transcriptome sequences of R. longipedicellatum, 102 primer sets were designed; 48 primer sets were successfully amplified, with 15 showing polymorphisms in 150 individuals from five extant populations of R. longipedicellatum. The number of alleles per locus ranged from four to 18, and the levels of observed and expected heterozygosity for the 15 loci varied from 0.255 to 0.913 an

SUBMITTER: Li T 

PROVIDER: S-EPMC6025811 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature