Characterization of polymorphic microsatellite loci for North American common greenbrier, Smilax rotundifolia (Smilacaceae).

ABSTRACT:

Premise of the study

Microsatellite markers were developed for Smilax rotundifolia (Smilacaceae), an understory vine widely distributed in eastern North America, to investigate genetic diversity and structure. Cross-amplification was tested in three congeneric species: S. china, S. riparia, and S. walteri.

Methods and results

A total of 6153 simple sequence repeat primer pairs were detected from the de novo-assembled transcriptome data (88,3962 contigs) of S. rotundifolia. Thirty-three polymorphic microsatellite loci were selected for further analysis among 96 individuals representing four natural populations of the species. The number of alleles ranged from two to 15, and 87.9% of the developed primer pairs could be cross-amplified in at l

SUBMITTER: Wang R 

PROVIDER: S-EPMC6025819 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature