CTCF-Binding Elements Mediate Accessibility of RAG Substrates During Chromatin Scanning.

ABSTRACT: RAG endonuclease initiates antibody heavy chain variable region exon assembly from V, D, and J segments within a chromosomal V(D)J recombination center (RC) by cleaving between paired gene segments and flanking recombination signal sequences (RSSs). The IGCR1 control region promotes DJ_H intermediate formation by isolating Ds, J_Hs, and RCs from upstream V_Hs in a chromatin loop anchored by CTCF-binding elements (CBEs). How V_Hs access the DJ_HRC for V_H to DJ_H rearrangement was unknown. We report that CBEs immediately downstream of frequently rearranged V_H-RSSs increase recombination potential of their associated V_H far beyond that provided by RSSs alone. This CBE activity becomes particularly str

SUBMITTER: Jain S 

PROVIDER: S-EPMC6026039 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature