HuR-mediated SCN5A messenger RNA stability reduces arrhythmic risk in heart failure.

ABSTRACT:

Background

Downregulated sodium currents in heart failure (HF) have been linked to increased arrhythmic risk. Reduced expression of the messenger RNA (mRNA)-stabilizing protein HuR (also known as ELAVL1) may be responsible for the downregulation of sodium channel gene SCN5A mRNA.

Objective

The purpose of this article was to investigate whether HuR regulates SCN5A mRNA expression and whether manipulation of HuR benefits arrhythmia control in HF.

Methods

Quantitative real-time reverse-transcriptase polymerase chain reaction was used to investigate the expression of SCN5A. Optical mapping of the intact heart was adopted to study the effects of HuR on the conduction velocity and action potential upstroke in mice with myocardial infarct and HF after injection of AAV9 viral

SUBMITTER: Zhou A 

PROVIDER: S-EPMC6026071 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature