Role of Dorsal Striatum Histone Deacetylase 5 in Incubation of Methamphetamine Craving.

ABSTRACT:

Background

Methamphetamine (meth) seeking progressively increases after withdrawal (incubation of meth craving). We previously demonstrated an association between histone deacetylase 5 (HDAC5) gene expression in the rat dorsal striatum and incubation of meth craving. Here we used viral constructs to study the causal role of dorsal striatum HDAC5 in this incubation.

Methods

In experiment 1 (overexpression), we injected an adeno-associated virus bilaterally into dorsal striatum to express either green fluorescent protein (control) or a mutant form of HDAC5, which strongly localized to the nucleus. After training rats to self-administer meth (10 days, 9 hours/day), we tested the rats for relapse to meth seeking on withdrawal days 2 and 30. In experiment 2 (knockdown), we inject

SUBMITTER: Li X 

PROVIDER: S-EPMC6026084 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature